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At specific time points post wounding, animals were humanely euthanised via CO 2 inhalation and the wounds were harvested for biochemical or histological analysis

Native NMT1-ribosome complex isolation A plasmid for transient expression of NMT1 was generated by inserting the coding sequence of NMT1 which was amplified from cDNA into a pcDNA5/FRT/TO mammalian expression vector (Thermo Fisher Scientific) that was modified to contain a N-terminal 3xFLAG-3C cleavage site tag via restriction cloning

Curr Biol (2018) 28(15):238899.e5

In the presence of even 6 mM TCEP hydrochloride, Hg 2+ prevents NF-B-DNA binding at concentrations as low as 20 M in binding reactions [1]

Schoeller EL, Schon S, Moley KH